Receptor-; TMA: tissue microarray; IHC: immunohistochemistry; OR: odds ratio; HR: hazard ratio; CEA: carcinoembryonic antigen; VGSCs: voltage-gated sodium channels; E2: -estradiol; DAB: 3,3-diaminobenzidine tetrahydrochloride; CI: confidence interval; DFS: disease no cost survival; OS: all round survival. Authors’ contributions JP and YF drafted the manuscript; XW, RZ and WJ participated in clinical data and paraffin-embedded specimens collection; JP, QO and YF played a key part in tissue microarray construction and immunohistochemistry examination; JP, YF and QZ carried out the information analysis; YF and ZL supervised the research program and edited the manuscript; DW, ZP and YF had considerable roles in the study style and manuscript assessment. All authors study and authorized the final manuscript. Author particulars 1 Department of Colorectal Surgery, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yatsen University Cancer Center, 651 Dongfeng Road East, Guangzhou 510060, Guangdong, P. R. China. two Department of Experimental Study, Sun Yat-sen University Cancer Center, Guangzhou 510060, Guangdong, P. R. China. three Division of Statistics, School of Public Well being, Guangzhou Medical University, Guangzhou 510182, Guangdong, P. R. China. Acknowledgements We deeply appreciate the support from all colleagues on the Department of Colorectal Surgery at Sun Yat-sen University Cancer Center, who’ve worked in getting the tissue specimens and offered it towards the present study. The authors would like to thank the two pathologists, Shixun Lu and Peng Li from Department of Pathology at Sun Yat-sen University Cancer Center for the TMA-IHC evaluation. Competing interests The authors declare that they have no competing interests. The authenticity of this short article has been validated by uploading the important raw information onto the Analysis Data Deposit public platform (http://www.37700-64-4 manufacturer researchdata.893567-09-4 Chemscene org.PMID:23724934 cn), with all the Approval Number as RDDB2017000048. Availability of information and materials The datasets analysed for the duration of the current study had been offered in the corresponding author on affordable request. Anyone who is keen on the information ought to make contact with [email protected] and [email protected]. Consent for publication Not applicable.Ethics approval and consent to participate This study was undertaken in accordance using the ethical standards from the World Health-related Association Declaration of Helsinki. Informed consents ahead of initial remedy for working with tissue samples were requested and the study approval was obtained from the Institutional Research Ethics Committee of Sun Yat-sen University Cancer Center (Approval Number: GZR-2016-071). Funding This function was supported by a grant in the Sun Yat-sen University Clinical Analysis 5010 System (Grant No. 2015024), a grant of Guangzhou Science and Technologies Plan Projects (Health Medical Collaborative Innovation Plan of Guangzhou) (Grant No. 201400000001-4) and a grant of National Organic Science Foundation of China (Grant No. 81772595). Received: 15 February 2017 Accepted: 21 OctoberReferences 1. Chen W, Zheng R, Baade PD, Zhang S, Zeng H, Bray F, et al. Cancer statistics in China, 2015. CA Cancer J Clin. 2016;66(2):1152. 2. Xu J, Qin X. Specialist consensus on robotic surgery for colorectal cancer (2015 edition). Chin J Cancer. 2016;35:23. three. Xu RH, Shen L, Li J, Xu JM, Bi F, Ba Y, et al. Specialist consensus on upkeep therapy for metastatic colorectal cancer in China. Chin J.